Fitc-conjugated pac-1

WebAug 2, 2010 · The molar ratio of FITC and polymer taken was 1:20. The labeled polymer solution was dialysed against PBS (pH 7.4) for 24 h and then distilled water for 24 h (MWCO 500 for PEI 600, MWCO 1000 for PEI 1800, MWCO 3500 for PEI 25k) until free FITC could not be detected by TLC (chloroform/methanol = 1/1, v/v). ... After conjugation with TA, … WebNov 19, 2014 · FITC-RGD2, FITC-3P-RGD2, and FITC-Galacto-RGD2 were prepared, and their integrin αvβ3/αvβ5 binding affinity was determined using the displacement assay against (125)I-echistatin bound to U87MG glioma cells. IC50 values of FITC-Galacto-RGD2, FITC-3P-RGD2, and FITC-RGD2 were calculated to be 28 ± 8, 32 ± 7, and 89 ± 17 nM, …

FITC anti-human CD41/CD61 Antibody anti-CD41/CD61 - PAC-1

Webphore-conjugated monoclonal antibodies (mAbs): phycoerythrin (PE)-conjugated GPIb (CD42b, Clone HIP1), allophycocyanin (APC)-conjugated P-selectin (CD62P, Clone Psel.KO2.3) (both eBioscience, San Diego, CA, USA), and fluorescein isothiocyanate (FITC)-conjugated PAC-1 (Becton Dickinson Bioscience, San Jose, CA, USA). WebConjugation: FITC: Tested applications: FACS, ICC, IF, IP: Immunogen: Human platelets: Target: PAC-1 (FITC). The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 … greenland holdings china https://imagesoftusa.com

IHC-plus™ ADCYAP1R1 / PAC1 Receptor Polyclonal Antibody …

WebClone HIT3a. Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Whole blood was stained with either FITC Mouse IgG2a, κ Isotype Control (Cat. No. 555573; dashed line histogram) or FITC Mouse Anti-Human CD3 (Cat. No. 561802/555539; solid line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. … Weball FITC has dissolved. Label vial “20:1 FITC”. The solution should be freshly prepared before each conjugation and used within 5 minutes (See Application Note 6). The "20:1 FITC" solution is further used to prepare the "5:1" and "10:1" solutions. 5. Prepare the required dilution of FITC in 0.1 M carbonate-bicarbonate buffer as directed in ... WebConjugate: FITC. Order Details. View Available Conjugates. Catalog# & Conjugate Size Price; Alexa Fluor 350 NBP2-62201AF350: Alexa Fluor 405 NBP2-62201AF405 ... (PAC-1) [FITC] and receive a gift card or discount. Submit a Review. Bioinformatics. Gene Symbol: ITGA2B Popular Products ... flyff summon monster command

Fig. 1 a Anti-human fibrinogen antibody conjugated with FITC …

Category:Platelet Activation - BD Biosciences

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Fitc-conjugated pac-1

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Web(340507) BD™ FITC Mouse Anti-Human PAC-1 - BD Biosciences - CiteAb. 156 citations – A FITC-conjugated mouse monoclonal antibody, raised against Proteasome assembly chaperone 1 (Human), supplied by BD Biosciences. ... Conjugate FITC Target Proteasome assembly chaperone 1 (Human) UniProt O95456 - PSMG1_HUMAN. Gene PSMG1 … WebDec 1, 2024 · The mono-conjugated FITC-insulin (22%) with unlabeled insulin prepared from 0.25:1 molar ratio (Fig. 3 A), tri-conjugated FITC-insulin (84%) with little amount of di-conjugate prepared from 4:1 molar ratio (Fig. 2 D) and the unlabeled insulin were chosen for the transport study. The percentage amount of mono-conjugate and tri-conjugate …

Fitc-conjugated pac-1

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WebThe PAC-1 Antibody FITC has been validated for the following applications: Flow Cytometry. Flow cytometry of PAC-1 in PHA-activated human peripheral blood. Sample was incubated with PAC-1 monoclonal antibody (Product # MA5-28564) conjugated to FITC. WebColorectal cancer (CRC) is the third most common form of cancer worldwide, with men having a slightly higher incidence rate compared to women. Risk for developing colorectal cancer usually increases with age and can be attributed to inherited genetic mutations along with other comorbidities such as diabetes, IBD, obesity, poor diet, and an ...

WebCells were blocked with 1% BSA for 30 min and incubated for 60 min with 1:200 dilution of FITC-conjugated rabbit polyclonal anti-C1q antibody (Abcam, Cambridge, MA) and 0.4 μg/ml polyclonal, C-terminal specific rabbit anti-AQP4 antibody (Santa Cruz Biotechnology). Cells were incubated for 30 min with 5 μg/ml goat anti-rabbit IgG-conjugated ... WebIn Fig. 1a, we used FITC- conjugated anti-human fibrinogen antibody to highlight intercellular reactivity (white dots, red arrow). The round structures (blue arrow) seem to be a series of TTS ...

WebSpecifications. Name. Anti-PAC-1 Antibody [PAC-1] (FITC) Description. Mouse monoclonal [PAC-1] antibody to PAC-1 (FITC). Specificity. This antibody recognizes an extracellular activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation ... WebConjugation: FITC: Tested applications: FACS, ICC, IF, IP: Immunogen: Human platelets: Target: PAC-1 (FITC). The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation.

Websupplied, and the antibody subclass for each FITC-conjugated antibody. The amount of antibody provided is sufficient for 25 immunostaining reactions using a 1 mL working solution per reaction. Antibody Concentration Amount Subclass Anti-His(C-term)-FITC 1 mg/mL 50 μl (25 reactions)† IgG 2b Anti-myc-FITC 1 mg/mL 50 μl (25 reactions)† IgG 1

WebApr 7, 2024 · And then, they were thoroughly washed, stained orderly with the vinculin antibody (1:500) overnight at 4 °C, the FITC-conjugated goat-anti-rabbit secondary antibody (1:5000) for 1 h, rhodamine-conjugated phalloidin (1:250) for 1 h, and DAPI (1:5000) for 5 min at room temperature. greenland holidays and celebrationsWebJun 1, 2002 · On human platelets, the high affinity conformation of alphaIIbbeta3 is detected by the monoclonal antibody (mAb), PAC-1. However, a reagent with binding specificity to high affinity mouse alphaIIbbeta3 has not been described so far. ... JON/A was conjugated with fluorescein isothiocyanate (JON/A(FITC)) or with R-phycoerythrin (JON/A(PE)) and ... flyff swimsuitWebFeb 16, 2024 · The blood platelets were distinguished based on the expression of CD61. For each sample, 10,000 CD61-positive objects (platelets) were acquired. For the assessment of P-selectin expression, samples were labeled with fluorescently conjugated monoclonal antibody PAC-1-FITC. Results are shown as the percentage of platelets binding PAC … flyff suitWebFITC Conjugated Anti-PAC-1 Monoclonal Antibody (Clone:PAC-1) Share: Figure 1: Surface staining of PAC-1 on PHA-activated (1 hour) human peripheral blood with anti-PAC-1 (PAC-1) FITC. flyff sweet medicationWebMutated KRAS proteins are often constitutively active, resulting in the dysregulated growth and transformation pathways that promote pancreatic cancer growth, survival, and metastasis. Proteintech’s KRAS antibody is a favorite among researchers worldwide, cited in nearly 100 publications. Immunohistochemical analysis of paraffin-embedded ... greenland holdings corp stock chartWebAug 1, 2003 · MFI of PAC-1-FITC was assessed in 5 whole blood samples immediately, 15, 30, and 60 min after antibody labelling with PAC-1 and CD42b MoAbs. greenland homeã‚â® southwest shower curtainWebFITC Protocols Fluorescein Isothiocyanate (FITC) - conjugated Antibodies GFP Protocols › Immunoprecipitation Protocols › Membrane Protein Protocols › Protein Expression Protocols › Protein Isolation Protocols › Protein Purification Protocols › SDS PAGE Protocols › greenland holidays from uk