Hieffclone
Web12 de nov. de 2024 · 一种pbiflu荧光标签表达载体的制备方法及其应用 技术领域 1.本发明属于分子生物学技术领域，具体涉及一种pbiflu荧光标签表达载体的制备方法及其应用。 背景技术： 2.黄色荧光蛋白(yellow fluorescent protein，yfp)是将绿色荧光蛋白(gfp)第203为苏氨酸突变体为色氨酸，可以稳定生色团在激发状态的偶极矩 ... Web1 de dez. de 2024 · Introduction. Mutations Duchenne muscular dystrophy (DMD) is hereditary disease carried by the X chromosome, which driven by the mutation in DMD gene [1,2].DMD gene encodes Dystrophin protein that maintains to support and stabilize the structure and function of muscle tissue. The mutation of DMD gene lead to an absence …
Hieffclone
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WebDownload scientific diagram High G9a level is correlated with high Plk1 level in CRC. (A) Representative photomicrographs of IHC staining of G9a and Plk1 in CRC tissues and corresponding ... WebHSpHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) according to the manufacturer's instructions, and transformed into BL21 (DE3) competent cells. The correct clones were screened by primer P7/P8 (Table I) and colony PCR protocol in the above steps. inoculated into 3 ml fresh LB
WebHSpHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) according to the manufacturer's instructions, and transformed into … WebHieffClone®ZeroTOPO-TASimpleCloningKit不含多克隆酶切位点(MCS)，在引入后续酶切位点进行酶切操作时，不会受到 MCS位点的影响，从而增加酶切效率以及克隆成功率。 …
Web翌圣生物以工具酶为核心,研发生产销售pcr,qpcr,高保真酶,热启动酶,一步法快速克隆试剂盒,一步法快速支原体检测试剂盒等分子、蛋白、细胞、免疫等3000多种试剂及试剂盒,现有科 … Web20 de jan. de 2024 · All plasmids were constructed using HieffClone One Step Cloning Kit (YEASEN, 10911). Point mutations of c-Myc (L297A, A321del, L333A, R346A, S347A, …
Web产品描述. 本试剂盒是利用拓扑异构酶（Topoisomerase）高效快速连接DNA片段的原理进一步研发而成，与传统的T4连接酶相比，具有以下优势：1）快速，仅需1-5分钟即可完成 …
Web丁香通为您提供HieffClone零背商品详情介绍：价格：￥530，货号：10907ES20，品牌：Yeasen翌圣，产地：上海，详见丁香通HieffClone零背商品详情页； cumberland tree service crossville tncumberland trucking ephrataWeb20 de jan. de 2024 · All plasmids were constructed using HieffClone One Step Cloning Kit (YEASEN, 10911). Point mutations of c-Myc (L297A, A321del, L333A, R346A, S347A, E351A, V361A, and Q365A) were generated using Hieff Mut Site-Directed Mutagenesis Kit (YEASEN, 11003ES10). cumberland treatment center virginiaWebHieffClone®ZeroTOPO-BluntCloningKit零背景TOPO-Blunt平末端克隆试剂盒 10909ES20 20T 625.00 产品描述 本试剂盒是利用拓扑异构酶（Topoisomerase）高效快速连接DNA … east texas scoreboardWeb29 de jun. de 2024 · The DNA fragment of TurboID was cloned into this digested vector by a HieffClone Plus multi One-Step Cloning Kit (10912ES10, Yesen, China). The TurboID fragment was amplified by PCR from a commercial gene (USA126049; Addgene, Watertown, MA, USA). The sequences of the primers were listed in Table S1. east texas scorpion speciesWebCN114107369A CN202411458461.XA CN202411458461A CN114107369A CN 114107369 A CN114107369 A CN 114107369A CN 202411458461 A CN202411458461 A CN 202411458461A CN 114107369 A CN114107369 A CN 114107369A Authority CN China Prior art keywords mcs pgl vector primer reaction Prior art date 2024-12-02 Legal status … east texas school gas explosionWeb6 de mar. de 2024 · The cloning backbone PL451 and the three segments of CVA10 carrying overlapping end (15–20 bp) were amplified and seamlessly linked together in … east texas rolling hills